Weaving together all the elements you've outlined, "Remember ":Everything that occurs in the opening segment of your story must be linked to something that happens later in the story."
Karen Wiesner uses an approach like this Character leads to Event leads to Conflict leads to Backstory leads to Goals leads to Motivation leads to Choice leads to Resolution..
Doin this with the main characters will form a pattern for the story. The characters must belong in the setting. The plot becomes an element that drives the story as the characters drive the plot. Having all your elements work cohesively is what makes a story the reader won't put down. The story can become one that is read and reread with the reader learning something new every time the book is read.